Victorian Homicide Squad officers went to the Gold Coast home of John Nikolic on Tuesday and removed undisclosed items.
Mr Nikolic is the brother of prominent jockey Danny Nikolic who is Mr Samba's former son-in-law.
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Police said in a statement they executed a search warrant at a Robina home and seized a number of items.
Victorian detectives also flew to Sydney on Tuesday to conduct further interviews.
The latest development in the Samba case followed an emotional plea for information on the murder from his daughter on Monday.
Victoria Samba said she was unaware of any enemies her father may have had, describing him a "a strong man and the bravest person I knew".
She said she had no knowledge of any link her father is rumoured to have had to the drug trade.
"It is extremely heartbreaking to know he was terrified in his last moments of life, running from a person with a gun."
She described the killer as "cold-hearted and dangerous" and pleaded for public help to solve his murder.
Mr Samba, 60, a former horse trainer, was shot dead on a busy Melbourne street on February 27.
Police say they are pursuing a number of avenues of inquiry.
Among them has been an earlier visit to Queensland to interview John Nikolic.
Earlier in the investigation Danny Nikolic, who was married to Ms Samba, voluntarily went to police in Melbourne to be interviewed.
Mr Samba raced a number of horses, including some in partnership with Sydney businessman and accused killer Ron Medich.
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